a dot plot if you were looking at scatter or fluorescent parameters on both the X- and Y-axes). FlowJo will create an Overlay based on the type of plot that was last used in the Graph Window for that population (e.g. Then drag a second population on top of the first one. Creating an Overlay in the Layout Editor.Ĭreating Overlays in FlowJo v10 is fast and easy. To create an overlay, drag a population from the Workspace into the Layout Editor. Histogram overlays have the added benefit of being displayed offset to one another, the controls of which are discussed further below. Find rare populations, identify various subsets, define cytokine expressing cells, compare controls to experimentals and many other functions with overlays.įlowJo vX allows you to create both univariate ( histograms, cumulative distribution function) and bivariate ( dot, pseudocolor, density, contour, and zebra plots) Overlays within the Layout Editor. Differences between populations within a single experiment or amongst groups of experiments are easily seen. Overlaying univariate or bivariate plots is a powerful visual tool to for cytometric analyses. Compare a control to an experimental set, evaluate the accuracy of gates, find high or low expressors, or a perform a myriad of other analyses using Overlays in FlowJo v10.
